Research Article Summary: 2 Billion Year mafic rocks with Microbial Life

Subsurface Microbial Colonization at Mineral-Filled Veins in 2-Billion-Year-Old Mafic Rock from the Bushveld Igneous Complex, South Africa,” focuses on the examination of microbial life in ancient igneous rocks from the Bushveld Igneous Complex in South Africa. The study investigates the possibility of long-term microbial survival in stable subsurface environments over geological timescales.

1000-fold magnification of lenticular microbial cells stained with SYBR Green 1. SYBR Green I (SG) is an extremely sensitive asymmetrical cyanine dye used as a nucleic acid stain in molecular biology. SYBR Green I binds to DNA. The resulting DNA-dye-complex best absorbs 497 nanometer blue light (λmax = 497 nm) and emits green light (λmax = 520 nm). The stain preferentially binds to double-stranded DNA, but will stain single-stranded (ss)DNA with lower performance. SYBR Green can also stain RNA with a lower performance than ssDNA.
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